Journal of research in sport medicine and technology

The effect of acute glutamine supplementation along with intermittent exercise on Hsp70 and blood lactate response of club soccer players

Document Type : Original Article

Authors

Sajad karami 1
Hamid Rajabi 2
Majid Kashef 1
Mohammad Ali Gharaat 3

1 Department of Physical Education and sport Science, Teacher Training Shahid Rajaee University of Tehran.

2 Department of Physical Education and sport science, Kharazmi University of Tehran.

3 Department of Physical Education, Farhangian University, Tehran, Iran.

10.22034/jsmt.2026.7736
Abstract
Introduction and purpose: The present study was conducted with the aim of investigating the effect of acute glutamine supplementation along with intermittent exercise on Hsp70 and blood lactate response of club football players.
Methodology: 32 players of the Tehran Railway team were selected and randomly divided into four groups: control, supplement, supplement-sports activity, and sports-activity. Subjects participated in daily exercises before conducting the study, and only two complementary groups - sports activity and sports activity - performed the periodic protocol. Blood samples were taken at baseline, pre-test, post-test and 90 minutes after the test. The supplement and placebo were consumed in an amount of 0.5 g/kgBW and a volume of 5 ml/kgBW one hour before the periodic protocol. The interval protocol included 3 stages of 20-minute running with an intensity of 80% of the maximum heart rate and a 5-minute rest of walking between the stages. HSP72 and blood lactate levels were measured using ELISA and enzymatic methods, respectively. The data were analyzed using the analysis of variance test with repeated measures and the between-group factor at the P≤0.05 level.
Findings: Glutamine supplement alone and together with exercise stimulate the response of HSP72 and the combination of supplement and exercise leads to a greater response of HSP72 and lactate changes did not play a role in this response.
Discussion and conclusion: It seem that taking glutamine supplements in the long term creates more adaptations, which of course should be studied.

Keywords

HSP72
Lactate
glutamine supplement
Soccer Players
interval exercise
Subjects
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  • Receive Date 27 February 2026
  • Revise Date 15 June 2026
  • Accept Date 21 June 2026
  • First Publish Date 21 June 2026
  • Publish Date 01 July 2026