دوره 21، شماره 26 - ( 10-1402 )
جلد 21 شماره 26 صفحات 29-18 |
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talebi-garakani E, kheradmand S, nasiri K. The Effect Of High Intensity Aerobic Training On Mitochondrial DNA Copy Number In Visceral Adipose Tissue Of Ovariectomized Rats Fed With High-Fat Diet. RSMT 2023; 21 (26) :18-29
URL: http://jsmt.khu.ac.ir/article-1-587-fa.html
URL: http://jsmt.khu.ac.ir/article-1-587-fa.html
خردمند شکوفه، طالبی گرکانی الهه، نصیری خدیجه. تأثیر تمرین هوازی شدید بر تعداد کپی DNA میتوکندری بافت چربی احشایی موش‌های صحرایی فاقد تخمدان و تغذیه شده با غذای پرچرب. پژوهش در طب ورزشی و فناوری. 1402; 21 (26) :18-29
دانشگاه مازندران ، UMZ.kheradmand@gmail.com
چکیده: (4253 مشاهده)
شیوع چاقی در زنان یائسه نسبت به مردان بیشتر است. کاهش استروژن هنگام یائسگی موجب اختلال در عملکرد میتوکندری می‌شود. هدف از پژوهش حاضر بررسی تأثیر تمرین هوازی شدید (HIT) بر تعداد کپی‌های ژنوم میتوکندری (mtDNAcn) و فاکتور رونویسی میتوکندری A (Tfam) در بافت چرب احشایی (VAT) موش‌های صحرایی اورکتومی (OVX) و تغذیه شده با غذای پرچرب (HFD) بود. 40 سر موش (8 هفته‌ای، 20±200 گرم) به 5 گروه:1) غذای نرمال (ND) 2) ND+OVX 3) HFD 4) HFD+ OVX5) HFD+OVX+HIT، تقسیم شدند. HIT به مدت 8 هفته/5 جلسه در هفته/42 دقیقه تمرین هوازی (85–80 درصد حداکثر اکسیژن مصرفی) اجرا شد. 48 ساعت پس از آخرین جلسه تمرین از VAT برای ارزیابی mtDNAcn و بیان ژن Tfam به روش qRT-PCR، نمونه‌برداری شد. از آزمون ANOVA و LSD جهت مقایسه تفاوت‌های بین گروهی استفاده شد. OVX و HFD منجر به تغییر معنادار بیان ژن Tfam نشد اما موجب کاهش معنادار mtDNAcn نسبت به گروه کنترل شد. HIT موجب افزایش معنادار بیان ژن Tfam نسبت به گروه کنترل و بیان ژن Tfam و mtDNAcn نسبت به گروه HFD+ OVX گردید؛ بنابراین HIT با افزایش mtDNAcn و بیان ژن Tfam موجب بهبود عملکرد میتوکندریایی در VAT موش‌های صحرایی OVX و تغذیه شده با HFD می‌شود.
نوع مطالعه: پژوهشي |
موضوع مقاله:
فیزیولوژی ورزشی
دریافت: 1402/3/16 | پذیرش: 1402/6/1 | انتشار: 1402/10/10
دریافت: 1402/3/16 | پذیرش: 1402/6/1 | انتشار: 1402/10/10
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