دوره 21، شماره 26 - ( 10-1402 )
جلد 21 شماره 26 صفحات 29-18 |
برگشت به فهرست نسخه ها
دانشگاه مازندران ، UMZ.kheradmand@gmail.com
چکیده: (1204 مشاهده)
شیوع چاقی در زنان یائسه نسبت به مردان بیشتر است. کاهش استروژن هنگام یائسگی موجب اختلال در عملکرد میتوکندری می‌شود. هدف از پژوهش حاضر بررسی تأثیر تمرین هوازی شدید (HIT) بر تعداد کپی‌های ژنوم میتوکندری (mtDNAcn) و فاکتور رونویسی میتوکندری A (Tfam) در بافت چرب احشایی (VAT) موش‌های صحرایی اورکتومی (OVX) و تغذیه شده با غذای پرچرب (HFD) بود. 40 سر موش (8 هفته‌ای، 20±200 گرم) به 5 گروه:1) غذای نرمال (ND) 2) ND+OVX 3) HFD 4) HFD+ OVX5) HFD+OVX+HIT، تقسیم شدند. HIT به مدت 8 هفته/5 جلسه در هفته/42 دقیقه تمرین هوازی (85–80 درصد حداکثر اکسیژن مصرفی) اجرا شد. 48 ساعت پس از آخرین جلسه تمرین از VAT برای ارزیابی mtDNAcn و بیان ژن Tfam به روش qRT-PCR، نمونه‌برداری شد. از آزمون ANOVA و LSD جهت مقایسه تفاوت‌های بین گروهی استفاده شد. OVX و HFD منجر به تغییر معنادار بیان ژن Tfam نشد اما موجب کاهش معنادار mtDNAcn نسبت به گروه کنترل شد. HIT موجب افزایش معنادار بیان ژن Tfam نسبت به گروه کنترل و بیان ژن Tfam و mtDNAcn نسبت به گروه HFD+ OVX گردید؛ بنابراین HIT با افزایش mtDNAcn و بیان ژن Tfam موجب بهبود عملکرد میتوکندریایی در VAT موش‌های صحرایی OVX و تغذیه شده با HFD می‌شود.
نوع مطالعه: پژوهشي |
موضوع مقاله:
فیزیولوژی ورزشی
دریافت: 1402/3/16 | پذیرش: 1402/6/1 | انتشار: 1402/10/10
دریافت: 1402/3/16 | پذیرش: 1402/6/1 | انتشار: 1402/10/10
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