The present study aimed to examine the effects of FIFA 11+ comprehensive warm-up program for referees on dynamic balance among male Iranian football referees and assistant referees. Fifty-two football referees and assistant referees who had no previous injury voluntarily participated in the present study. They were randomly assigned into an intervention group and a control one (26 participants each group). The intervention participants took part in a 17-week FIFA 11+ comprehensive warm-up program for referees while the control group performed their usual warm-up trainings during a half season. In order to estimate the dynamic balance of the referees in the pre-test and post-test, Y Balance Test was used. The collected data were analyzed using Descriptive statistics and ANOVA. The results of the present study indicated that the intervention and the control groups were significantly different in terms of dynamic balance in anterior and posteromedial directions and also the referees’ overall balance score before and after the study. However, no significant between them was observed in posteriolateral direction. According to the findings of the present study, it can be concluded that the FIFA 11+ comprehensive warm-up program for referees could improve the referees’ and the assistant referees’ dynamic balance.