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Shahid Rajaee Teacher Training University , salehpour57@gmail.com
Abstract:   (694 Views)
Introduction: The increase of iron due to aging affects the process of ferroptosis, and the final product of lipid peroxidation is MDA, and GPX4 is the central and negative regulator of ferroptosis. It seems that sports activities can moderate this aging pathway by reducing lipid peroxidation. Therefore, the aim of the research was to determine the effect of eight weeks of endurance training on the expression of GPX4 and MDA in the soleus muscle of aged male rats.
Materials and methods: This study is of an experimental type. 20 healthy rats (with an average age of 20 months and an average weight of 373 grams) were divided into two groups of 10: training group and control group. Training was performed incrementally for eight weeks, five sessions per week on a rodent-specific treadmill. 48 hours after the last training session, the soleus muscle of the mice was surgically removed and the amount of GPX4 was measured by the gene expression method and the amount of MDA was measured by the ELISA method. For data analysis, the Shapiro-Wilk test was used to measure the normal distribution of the data, and the Levine test was used to determine the homogeneity of variances, and the independent t test was used to determine the difference between two groups, and the De Cohen test was used to measure the effect size, and the calculations Statistics were done by Prism Graph Pad software version 8.
Findings: There was no significant difference in the level of GPX4 gene expression in the soleus muscle of the training group rats compared to the control group rats (p=0.4079), but the MDA level in the training group decreased significantly compared to the control group (p=0.0028).
Conclusion: According to the findings of the present research, it seems that the effect of endurance training in this research shows the reduction of ferroptosis. The lack of significant difference in GPX4 gene expression shows that endurance training with this intensity does not affect it.
Type of Study: Research | Subject: sport physiology
Received: 2023/06/3 | Accepted: 2023/10/14

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