Compare of Two Aerobic Training Methods on SOD and TNF-α levels in the Heart Tissue of Type 2 Diabetic Rats

Barzegari, Ali; Naghibi, Saeid; Safayi, Samaneh; Dashti Khovidaki, Mohammadhasan; Salehi, Anahita

Volume 20, Issue 24 (3-2023) RSMT 2023, 20(24): 43-56 | Back to browse issues page

‎ 20.1001.1.22520708.1401.20.24.4.9

Mendeley

Zotero

RefWorks

Barzegari A, Naghibi S, Safayi S, Dashti Khovidaki M, Salehi A. Compare of Two Aerobic Training Methods on SOD and TNF-α levels in the Heart Tissue of Type 2 Diabetic Rats. RSMT 2023; 20 (24) :43-56
URL: http://jsmt.khu.ac.ir/article-1-536-en.html

Compare of Two Aerobic Training Methods on SOD and TNF-α levels in the Heart Tissue of Type 2 Diabetic Rats

Ali Barzegari

, Saeid Naghibi

, Samaneh Safayi

, Mohammadhasan Dashti Khovidaki

, Anahita Salehi

Payame Noor University , ali_barzegari@pnu.ac.ir

Abstract: (2138 Views)

The purpose of this study was to compare two methods of aerobic exercise on the levels of SOD and TNF-α in the heart tissue of diabetic rats. 40 male Wistar rats aged ten weeks were randomly divided into four groups of 10: healthy control, diabetes, diabetes+MIT and diabetes+HIT. The moderate intensity training group ran 5 sessions per week for 8 weeks on the treadmill for 60 minutes at a speed of 25 meters per minute, and the high intensity training group also 5 sessions per week for 8 weeks with a training time of 60 minutes and at a speed of 34 meters per minute. The heart tissue of rats was examined to determine the amount of changes in SOD and TNF-α using ELISA method. To investigate the difference between groups of variables, one-way analysis of variance and Tukey's post hoc statistical tests were used at the significance level of P≥0.05. The results showed that there was a significant difference in the levels of TNF-α and SOD in the heart tissue of rats among the four research groups (P≤0.001). The levels of TNF-α in the HIT training group also showed a significant decrease compared to the diabetic group (P=0.001), While these changes in the MIT exercise group compared to the diabetes group was a non-significant decrease (P=0.117), the amount of SOD levels between the MIT and HIT exercise groups also showed a significant increase compared to the diabetic group (P=0.001). It seems, aerobic exercise with different intensities can improve SOD and TNF-α levels. Although the intensity of exercise in periodic exercise has been an effective factor in changing the values of both variables.

Keywords: Diabetes, MIT, HIT, SOD, TNF-α.

Full-Text [PDF 925 kb] (918 Downloads)

Type of Study: Research | Subject: sport physiology
Received: 2022/10/2 | Accepted: 2023/03/6 | Published: 2023/03/6

References

1. 1. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes research and clinical practice. 2010;87(1):4-14. [DOI:10.1016/j.diabres.2009.10.007]

2. Sharma M, Afaque A, Dwivedi S, Jairajpuri ZS, Shamsi Y, Khan MF, et al. Cichorium intybus attenuates streptozotocin induced diabetic cardiomyopathy via inhibition of oxidative stress and inflammatory response in rats. Interdiscip Toxicol. 2019;12(3):111. [DOI:10.2478/intox-2019-0013]

3. Ahmed D, Sharma M, Kumar V, Bajaj HK, Verma A. 2β-hydroxybetulinic acid 3β-caprylate: an active principle from Euryale Ferox Salisb. seeds with antidiabetic, antioxidant, pancreas & hepatoprotective potential in streptozotocin induced diabetic rats. Journal of food science and technology. 2015;52(9):5427-41. [DOI:10.1007/s13197-014-1676-0]

4. Eizadi M, Haji Rasouli M, Khorshidi D. The Effect of 3 Months of Aerobic Training on TNF-α and Insulin Resistance in Obese Men with Type 2 Diabetes. Journal of Sport Biosciences. 2019;11(3):253-69.

5. Norouzpour M, Marandi SM, Ghanbarzadeh M, Zare Mayavan AA. The effect of combined training on serum concentrations of inflammatory cytokines and factors associated with metabolic syndrome in elderly women with fatty liver. Journal of Sport and Exercise Physiology. 2022;15(2):64-75. [DOI:10.52547/joeppa.15.2.64]

6. Wagenmakers AJ, Pedersen BK. The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. Essays in biochemistry. 2006;42:105-17. [DOI:10.1042/bse0420105]

7. Wenning P, Kreutz T, Schmidt A, Opitz D, Graf C, Voss S, et al. Endurance exercise alters cellular immune status and resistin concentrations in men suffering from non-insulin-dependent type 2 diabetes. Experimental and Clinical Endocrinology & Diabetes. 2013;121(08):475-82. [DOI:10.1055/s-0033-1343395]

8. Sabouri M, Hatami E, Pournemati P, Shabkhiz F. Inflammatory, antioxidant and glycemic status to different mode of high-intensity training in type 2 diabetes mellitus. Molecular Biology Reports. 2021;48(6):5291-304. [DOI:10.1007/s11033-021-06539-y]

9. Pandey KB, Mishra N, Rizvi SI. Protein oxidation biomarkers in plasma of type 2 diabetic patients. Clinical biochemistry. 2010;43(4-5):508-11. [DOI:10.1016/j.clinbiochem.2009.11.011]

10. Davari S, Talaei SA, Alaei H. Probiotics treatment improves diabetes-induced impairment of synaptic activity and cognitive function: behavioral and electrophysiological proofs for microbiome-gut-brain axis. Neuroscience. 2013;240:287-96. [DOI:10.1016/j.neuroscience.2013.02.055]

11. Kennedy A, Nirantharakumar K, Chimen M, Pang TT, Hemming K, Andrews RC, et al. Does exercise improve glycaemic control in type 1 diabetes? A systematic review and meta-analysis. PloS one. 2013;8(3):e58861. [DOI:10.1371/journal.pone.0058861]

12. Brocardo PS, Boehme F, Patten A, Cox A, Gil-Mohapel J, Christie BR. Anxiety-and depression-like behaviors are accompanied by an increase in oxidative stress in a rat model of fetal alcohol spectrum disorders: Protective effects of voluntary physical exercise. Neuropharmacology. 2012;62(4):1607-18. [DOI:10.1016/j.neuropharm.2011.10.006]

13. Bloomer RJ, Fisher-Wellman KH. Blood oxidative stress biomarkers: influence of sex, exercise training status, and dietary intake. Gender medicine. 2008;5(3):218-28. [DOI:10.1016/j.genm.2008.07.002]

14. Elosua R, Molina L, Fito M, Arquer A, Sanchez-Quesada J, Covas M, et al. Response of oxidative stress biomarkers to a 16-week aerobic physical activity program, and to acute physical activity, in healthy young men and women. Atherosclerosis. 2003;167(2):327-34. [DOI:10.1016/S0021-9150(03)00018-2]

15. Rami M, Habibi A. Effect of 6-weeks of endurance training on the activity of superoxide dismutase and glutathione peroxidase enzymes in the hippocampus of experimental diabetic male Wistar rats. SSU_Journals. 2018;26(6):483-94.

16. Vinetti G, Mozzini C, Desenzani P, Boni E, Bulla L, Lorenzetti I, et al. Supervised exercise training reduces oxidative stress and cardiometabolic risk in adults with type 2 diabetes: a randomized controlled trial. Scientific reports. 2015;5(1):1-7. [DOI:10.1038/srep09238]

17. Pereira AdS, Spagnol AR, Luciano E, Leme JACdA. Influence of aerobic exercise training on serum markers of oxidative stress in diabetic rats. Journal of physical education. 2016;27. [DOI:10.4025/jphyseduc.v27i1.2726]

18. Plaisance EP, Grandjean PW. Physical activity and high-sensitivity C-reactive protein. Sports medicine. 2006;36(5):443-58. [DOI:10.2165/00007256-200636050-00006]

19. Dashti Khavidaki MH, Faramarzi M, Azamian Jazi A, Banitalebi E. Effect of endurance training intensity (low, moderate and high) on the expression of skeletal muscle ATGL protein and serum levels of insulin and glucose in male diabetic rats. Scientific Journal of Kurdistan University of Medical Sciences. 2018;23(2):92-102. [DOI:10.29252/sjku.23.2.92]

20. Bo H-X, Li W, Yang Y, Wang Y, Zhang Q, Cheung T, et al. Posttraumatic stress symptoms and attitude toward crisis mental health services among clinically stable patients with COVID-19 in China. Psychological medicine. 2020:1-2. [DOI:10.1017/S0033291720000999]

21. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention & Rehabilitation. 2007;14(6):753-60. [DOI:10.1097/HJR.0b013e3281eacef1]

22. Fathi Zadeh M, alimoradi s. THE EFFECT OF AEROBICS EXERCISE ON DEPRESSION, ANXIETY, AND STRESS IN WOMEN WITH SUBCLINICAL HYPOTHYROIDISM. Nursing and Midwifery Journal. 2019;17(9):749-57.

23. Rognmo Ø, Hetland E, Helgerud J, Hoff J, Slørdahl SA. High intensity aerobic interval exercise is superior to moderate intensity exercise for increasing aerobic capacity in patients with coronary artery disease. European Journal of Preventive Cardiology. 2004;11(3):216-22. [DOI:10.1097/01.hjr.0000131677.96762.0c]

24. Asle Mohammadi Zadeh M, Kargarfard M, Marandi SM, Habibi A. Diets along with interval training regimes improves inflammatory & anti-inflammatory condition in obesity with type 2 diabetes subjects. Journal of Diabetes & Metabolic Disorders. 2018;17(2):253-67. [DOI:10.1007/s40200-018-0368-0]

25. Hopps E, Canino B, Caimi G. Effects of exercise on inflammation markers in type 2 diabetic subjects. Acta diabetologica. 2011;48(3):183-9. [DOI:10.1007/s00592-011-0278-9]

26. !!! INVALID CITATION !!! {Sabouri, 2021 #4140;Soleymani, 2022 #4491}.

27. Mallard AR, Hollekim-Strand SM, Coombes JS, Ingul CB. Exercise intensity, redox homeostasis and inflammation in type 2 diabetes mellitus. Journal of science and medicine in sport. 2017;20(10):893-8. [DOI:10.1016/j.jsams.2017.03.014]

28. Teixeira de Lemos E, Oliveira J, Páscoa Pinheiro J, Reis F. Regular physical exercise as a strategy to improve antioxidant and anti-inflammatory status: benefits in type 2 diabetes mellitus. Oxid Med Cell Longev. 2012;2012. [DOI:10.1155/2012/741545]

29. rajabi a, akbarnejad a, siahkohian M, yari m. The response of TNF-α, IL-6 serum levels and lipid profiles to two aerobic training frequencies with the same volume in obese middle-aged women with type 2 diabetic. Journal of Sport and Exercise Physiology. 2021;14(1):59-72. [DOI:10.52547/joeppa.14.1.59]

30. Ziccardi P, Nappo F, Giugliano G, Esposito K, Marfella R, Cioffi M, et al. Reduction of inflammatory cytokine concentrations and improvement of endothelial functions in obese women after weight loss over one year. Circulation. 2002;105(7):804-9. [DOI:10.1161/hc0702.104279]

31. FA G, Joseph J, George G. Serum total superoxide dismutase enzyme activity in type 2 diabetic patients with retinopathy in Mthatha region of the Eastern Cape Province of South Africa. Biomedical Research. 2017;28(2):532-8.

32. Farhangi N, Nazem F, Zehsaz F. Effect of endurance exercise on antioxidant enzyme activities and lipid peroxidation in the heart of the streptozotocin-induced diabetic rats. SSU_Journals. 2017;24(10):798-809.

33. Oliveira VNd, Bessa A, Jorge MLMP, Oliveira RJdS, de Mello MT, De Agostini GG, et al. The effect of different training programs on antioxidant status, oxidative stress, and metabolic control in type 2 diabetes. Applied Physiology, Nutrition, and Metabolism. 2012;37(2):334-44. [DOI:10.1139/h2012-004]

34. Mitranun W, Deerochanawong C, Tanaka H, Suksom D. Continuous vs interval training on glycemic control and macro‐and microvascular reactivity in type 2 diabetic patients. Scandinavian journal of medicine & science in sports. 2014;24(2):e69-e76. [DOI:10.1111/sms.12112]

35. de Moraes R, Van Bavel D, Gomes MdB, Tibiriçá E. Effects of non-supervised low intensity aerobic excise training on the microvascular endothelial function of patients with type 1 diabetes: a non-pharmacological interventional study. BMC Cardiovascular Disorders. 2016;16(1):1-9. [DOI:10.1186/s12872-016-0191-9]

36. De Sousa RAL. Brief report of the effects of the aerobic, resistance, and high-intensity interval training in type 2 diabetes mellitus individuals. International Journal of Diabetes in Developing Countries. 2018;38(2):138-45. [DOI:10.1007/s13410-017-0582-1]

37. Khayampour N, Peeri M, Azarbayjani MA, Delfan M. Effects of High Intensity Interval Training on the Gene Expression of PGC1-Α, CS and P-53 in the Cardiomyocyte of Male Obese Rats in Type 2 Diabetes. The Journal of Shahid Sadoughi University of Medical Sciences. 2021;28(11):3215-25. [DOI:10.18502/ssu.v28i11.5222]