High intensity interval training induced changes in the hepatic FGF-21 gene expression and serum  TNF-α in diabetic male rats

Fasihi Ramandi, E; Khaledi, N

doi:10.29252/jsmt.18.19.57

Volume 18, Issue 19 (7-2020) RSMT 2020, 18(19): 57-68 | Back to browse issues page

‎ 10.29252/jsmt.18.19.57

‎ 20.1001.1.22520708.1399.18.19.3.4

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Fasihi Ramandi E, Khaledi N. High intensity interval training induced changes in the hepatic FGF-21 gene expression and serum TNF-α in diabetic male rats. RSMT 2020; 18 (19) :57-68
URL: http://jsmt.khu.ac.ir/article-1-434-en.html

High intensity interval training induced changes in the hepatic FGF-21 gene expression and serum TNF-α in diabetic male rats

E Fasihi Ramandi

, N Khaledi ^*

, N.khaledi@khu.ac.ir

Abstract: (4020 Views)

Diabetes is a common metabolic disease. In diabetic patients glucose uptake is reduced and FGF-21 plays an important role in glucose uptake, alsoTNF-α is an inflammatory factor that increases in diabetes. The purpose of this study was to investigate the effect of 6 weeks of HIIT training on the gene expression FGF-21 in the liver and the serum TNF-α level of male diabetic rats. For this purpose, 48 Wistar rats were randomly divided into four groups Control, diabetes, high intensity interval training, and diabetes and high intensity interval training. For the induction of diabetes, peritoneal injection (Streptozotocin 50 mg/kg) was used. Training protocol including 10 set of 1-minute running (between each set of 2 minutes of rest) 3 sessions per week and was completed within 6 weeks. Finally, after the extraction of liver samples, the expression of the FGF-21 gene was measured by Real Time PCR and serum TNF-α level with ELISA kit.There was no significant change in expression of FGF-21 in any group, but the reduction of serum levels of inflammatory factors, such as TNF-α protein at the level of significance (p=0.05), and maintaining and improving the time to exhaustion, was shown by high intensity interval training (0.000).Likely the inflammatory factors of diabetes such as TNF-α have a deleterious effect on the expression and binding of FGF-21 (β-Klotho) cofactors And causes resistance to FGF-21 into various tissues of the body, such as the liver. Exercise can reduce inflammation caused by diabetes.

Keywords: FGF-21, TNF-α, High Intensity Interval Training, Diabetes, Time to Exhaustion

Full-Text [PDF 1193 kb] (838 Downloads)

Type of Study: Research |
Received: 2020/07/18 | Accepted: 2020/07/15 | Published: 2020/07/15

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