تاثیر مصرف حاد مکمل گلوتامین همراه با فعالیت ورزشی تناوبی بر پاسخ Hsp70 و لاکتات خون بازیکنان فوتبال باشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری فیزیولوژی ورزش، دانشگاه تربیت دبیر شهید رجایی.

2 استاد دانشگاه خوارزمی تهران.

3 استاد دانشگاه تربیت دبیر شهید رجایی.

4 استادیار گروه آموزش تربیت‌بدنی، دانشگاه فرهنگیان تهران.

10.22034/jsmt.2026.7736
چکیده
سابقه و هدف: مطالعه حاضر با هدف بررسی تاثیر مصرف حاد مکمل گلوتامین همراه با فعالیت ورزشی تناوبی بر پاسخ Hsp70 و لاکتات خون بازیکنان فوتبال باشگاهی انجام گرفت.
مواد و روش‌ها: 32 بازیکن تیم راه آهن تهران انتخاب و به صورت تصادفی به چهار گروه کنترل، مکمل، مکمل- فعالیت­ ورزشی و فعالیت­ ورزشی تقسیم شدند. آزمودنی­ها قبل از انجام مطالعه در تمرینات روزانه شرکت داشتند و فقط دو گروه مکمل- فعالیت ­ورزشی و فعالیت ­ورزشی، پروتکل تناوبی را انجام دادند. نمونه­­ خون پایه، پیش­آزمون، پس­آزمون و 90 دقیقه پس از آزمون گرفته شد. مکمل و دارونما به مقدار g/kgBW5/0 و حجم ml/kgBW5 یک ساعت قبل از پروتکل تناوبی مصرف شد. پروتکل تناوبی شامل 3 مرحله دویدن 20 دقیقه­ای با شدت 80% حداکثر ضربان قلب بیشینه و استراحت­ 5 دقیقه­ای پیاده­ روی بین مراحل بود. مقادیر HSP72 و لاکتات خون به ترتیب با استفاده از روش الایزا و آنزیماتیک سنجیده شد. داده­ها با استفاده از آزمون تحلیل واریانس با اندازه­های تکراری و عامل بین گروهی در سطح 05/0 P≤تحلیل شد.
یافته‌ها: مکمل گلوتامین به تنهایی و همراه با ورزش محرک پاسخ HSP72 بوده و ترکیب مکمل- فعالیت ­ورزشی منجر به پاسخ بزرگتری از HSP72 می­شود و در این پاسخ تغییرات لاکتات نقشی نداشته است.
نتیجه‌گیری: به نظر می­رسد که مصرف مکمل گلوتامین در طولانی مدت سازگاری­های بیشتری را ایجاد کند که البته باید مورد مطالعه قرار بگیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله English

The effect of acute glutamine supplementation along with intermittent exercise on Hsp70 and blood lactate response of club soccer players

نویسندگان English

Sajad karami 1
Hamid Rajabi 2
Majid Kashef 3
Mohammad Ali Gharaat 4
1 Department of Physical Education and sport Science, Teacher Training Shahid Rajaee University of Tehran.
2 Department of Physical Education and sport science, Kharazmi University of Tehran.
3 Department of Physical Education and sport Science, Teacher Training Shahid Rajaee University of Tehran.
4 Department of Physical Education, Farhangian University, Tehran, Iran.
چکیده English

Introduction and purpose: The present study was conducted with the aim of investigating the effect of acute glutamine supplementation along with intermittent exercise on Hsp70 and blood lactate response of club football players.
Methodology: 32 players of the Tehran Railway team were selected and randomly divided into four groups: control, supplement, supplement-sports activity, and sports-activity. Subjects participated in daily exercises before conducting the study, and only two complementary groups - sports activity and sports activity - performed the periodic protocol. Blood samples were taken at baseline, pre-test, post-test and 90 minutes after the test. The supplement and placebo were consumed in an amount of 0.5 g/kgBW and a volume of 5 ml/kgBW one hour before the periodic protocol. The interval protocol included 3 stages of 20-minute running with an intensity of 80% of the maximum heart rate and a 5-minute rest of walking between the stages. HSP72 and blood lactate levels were measured using ELISA and enzymatic methods, respectively. The data were analyzed using the analysis of variance test with repeated measures and the between-group factor at the P≤0.05 level.
Findings: Glutamine supplement alone and together with exercise stimulate the response of HSP72 and the combination of supplement and exercise leads to a greater response of HSP72 and lactate changes did not play a role in this response.
Discussion and conclusion: It seem that taking glutamine supplements in the long term creates more adaptations, which of course should be studied.

کلیدواژه‌ها English

HSP72
Lactate
glutamine supplement
Soccer Players
interval exercise
1.     Karami S, Kashef M, Mehri Alvar Y. Protective Effect of Glutamine by the Expression of HSP70 and Reduction of Cortisol on Exercise Induced Stress. J Arak Uni Med Sci 2015; 17 (10) :65-73. [in Persian]
2.     karami S, kashef M, gaeini A, rajabi H, amani M. The Effect of Glutamine Supplement on Changes in hsp72, Cortisol and Plasma Glucose after Exercise. Iranian Journal of Endocrinology and Metabolism. 2013; 15 (2) :166-173. [in Persian]
3.     Ranek MJ, Stachowski MJ, Kirk JA, Willis MS. The role of heat shock proteins and cochaperones in heart failure. Philosophica Transactions of the Royal Society B: Biologica Sciences. 2018; 373(1738): 2016053.
4.     Peng W, Zhang Y, Zheng M, Cheng H, Zhu W, Cao C-M, et al. Cardioprotection by CaMKII-Δb is mediated by phosphorylation of heat shock factor 1 and subsequent expression of inducible heat shock protein 70. Circulation research. 2010; 106(1):102- 10.
5.     Zhang C, Liu X, Miao J, Wang S, Wu L, Yan D, et al. Heat shock protein 70 protects cardiomyocytes through suppressing SUMOylation and nucleus translocation of phosphorylated eukaryotic elongation factor 2 during myocardial ischemia and reperfusion. Apoptosis: an international journal on programmed cell death. 2017;22(5):608.
6.     Park YH, Seo JH, Park J-H, Lee HS, Kim K-W. Hsp70 acetylation prevents caspasedependent/independent apoptosis and autophagic cell death in cancer cells. International Journal of Oncology. 2017; 51(2):573-8.
7.     Amani M, Gaeini A, Kashef M, Karami S. The Effect of One Session Continuous and Intermittent Aerobic Exercise on Blood Responses of HSP72, Cortisol and Creatine Kinase. Avicenna J Clin Med. 2013; 20 (3) :223-231. [in Persian]
8.     Mehri Alvar Y, Nazem F, Sayari A, Sayevand Z, Karami S, Erfani F. The effect of exercise on the immune response in different environmental conditions. EBNESINA. 2014; 16 (3) :23-31. [in Persian]
9.     Paulsen G, Lauritzen F, Bayer M. L, Kalhovde J.M, Ugelstad I, Owe S. G, Hallen J, et al. Sub cellular movement and expression of HSP27, β-crystallin, and HSP70 after two bouts of eccentric exercise in humans. J Appl Physiol. 2009; 107; 570-582.
10.  Ogata T, Yasuharu O, Kazuhiko H, Mitsuru H and Isao M. Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle. Am J Physiol Regul Integr Comp Physiol. 2009; 296;1557-1563.
11.  Kayani AC, Graeme LC, Malcolm JJ, McArdle A. Prolonged treadmill training increases HSP70 in skeletal muscle but does not affect age-related functional deficits. Am J Physiol Regul Inter Comp Physiol. 2008; 294; R568-R576.
12.  Hashemi Chashmi SZ, Dabidi Roshan V. The Effect of HIIT Exercise with Creatine Supplementation on Systemic Stress Protein: A Quasi-experimental Study of Gender and Type of Recovery. Razi J Med Sci. 2023; 30(3): 11-22. [in Persian]
13.  Alizadeh AM, Isanejad A, Sadighi S, Mardani M, Hassan ZM. High-intensity interval training can modulate the systemic inflammation and HSP70 in the breast cancer: a randomized control trial (Persian). J of Cancer Research & Clinical Oncology. 2019;145(10):2583-93. [in Persian]
14.  Pouzesh Jadidi J, Seifi-Skishahr F, Bolboli L, Pourrahim Ghoroughchi A. Effect of high intensity interval training on cardiomyocytes HSP70 and Caspase-3 gene expression levels in myocardial infarction male rat model. Applied Health Studies in Sport Physiology. 2022; 9(2): 1-11. [in Persian]
15.  Peake M, Suzuki K, Hordern M, Wilson G, Nosaka K and Coombes J. Plasma cytokine changes in relation to exercise intensity and muscle damage. j Appl Physiol. 2005; 95(5-6); 514-521.
16.  Fehrenbach E, Niess AM, Voelke K, Northoff H, and Mooren FC. Exercise intensity and duration affect blood soluble HSP72. Int J Sports Med. 2005 ;26 (7)552-557.
17.  Tavakkoli M, Saraie G. This study investigated the The effect of eight weeks of resistance training and glutamine supplementation on some hematological factors and maximum strength of male athletes. Researches in Sport Sciences and Medical Plants. 2021; 1 (4):39-47. [in Persian]
18.  Ehrenfried JA, Chen J, Li J, Evers M. Glutamine-mediated regulation of heat shock protein expression in intestinal cells. Surgery. 1995; 118:352.
19.  Chow A, Zhang R. Glutamine reduces heat shock-induced cell death on rat intestinal epithelial cells. J Nutr. 1998; 128:1296.
20.  Wischmeyer, P.E. Glutamine induces heat shock protein and protects against endotoxin shock in the rat. J Appl Physiol. 2001; 90: 2403-2410
21.  Marino LV, Pathan N, Meyer R, Wright V, Habibi P. Glutamine depletion and heat shock protein 70 in children with meningococcal disease. Clin Nutr. 2014; 33:915-21.
22.  Cruzat V, Macedo M, Noel K, Curi R, Newsholme P. Glutamine metabolism and immune function, supplementation and clinical translation. Nutrients. 2018; 23:10.
23.  Ko HM, Oh SH, Bang HS, Kang NI, Cho BH, Im SY, et al. Glutamine protects mice from lethal endotoxic shock via a rapid induction of MAPK phosphatase-1. J Immunol. 2009; 182:7957-62.
24.  Steinacker J. M, L. Wang, L. Gampert, Y. Liu, K. Nething & O. Prokopchuk. Effects of overexpression of heat shock protein 70 on energy metabolism. Med Sci Sports Exerc. 2005; 37, S455.
25.  Whelan SA & Hightower LE. Differential induction of glucose-regulated and heat shock proteins: effects of pH and sulfhydryl-reducing agents on chicken embryo cells. J Cell Physiol. 1985; 125, 251-258.
26.  Sancho,Adriana Carvajal. The acute effect of an energy drink on physical and cognitive performance of male athletes. Kinesiologia Slovenica. 2005; 11(2): 5-16.
27.  Bellmann KA, Wenz J, Radons V, Burkart R, Kleemann, Kolb H.Heat shock induces resistance in rat pancreatic islet cells against nitric oxide, oxygen radicals and streptozotocin toxicity in vitro. J Clin Invest .1995; 95: 2840- 2845.
28.  Liu Y, Mayr S, Opitz-Gress A, Zeller C, Lormes W, Baur S, Lehmann M, Steinacker JM. Human skeletalmuscle HSP70 response to training in highly trained rowers. J Appl Physiol. 1999; 86: 101-104.
29.  Karami S. The effect of glutamine supplementation and periodic training on HSP72 response in soccer players. Master of Science Degree in Sport Physiology, Shahid Rajaee University, Tehran, Iran. [in Persian]
30.  Daniel J, Peart a, Lars R, McNaughtona C, Adrian W, Midgley A, et al. Preexercise alkalosis attenuates the heat shock protein 72 response to a single-bout of anaerobic exercise. J Sci Med Sport. 14. 2011; 435–440.
31.  Karami S, Sdahidi F, Rajabi H, Golab F. Effect of 8-week resistance training on HIF-1α gene expression and Endothelial Progenitor Cells recall of blood after one session of resistance activity in elderly men. Sci J Iran Blood Transfus Organ. 2019; 16 (2) :124-132
32.   Gharaat M A, Kashef M, Jameie B, Rajabi H. Effect of endurance and high intensity interval swimming training on cardiac structure and Hand2 expression of rats. JSSU. 2017; 25 (9) :748-758
33.  Young-Oh Shin, Jae-Keun Oh, Hyun-Sik Sohna, Jun-Sang Bae, Mi-Young Lee, Jeong-Beom Lee, et al. Expression of exercise-induced HSP70 in long-distance runner’s leukocytes. J Thermal Biol. 2004; 29: 769–774.
34.  Oehler R, Pusch E, Zellner M. Cell type-specific variations in the induction of hsp70 in human leukocytes by feverlike wholebody hyperthermia. Cell Stress Chaperones. 2001; 6 (4): 306-15.
35.   Kordi N, Saydi A, Karami S, Bagherzadeh-Rahmani B, Marzetti E, Jung F, et al. Ferroptosis and Aerobic Training in Ageing: A Review. Clinical Hemorheology and Microcirculation. 1 Jan. 2024: 1 – 20.
36.  Samadi M, Nazem F, Gharaat MA. Designing the simulation training of taekwondo competition according to heart rate, blood lactate and rating of perceived exertion. Medicina Dello Sport. 2014; 67 (4): 581-592.
37.  Karami S, Shahidi F, Rajabi H, Golab F. Response of Endothelial Progenitor Cells and Expression of Angiogenic Cytokine Genes in Preconditioning with Resistance Training in Elderly Men. Iranian Journal of Endocrinology and Metabolism 2020; 22 (4) :337-348
38.  Karami S, Ramezani A. Adaptation in Response of Excitation and Inhibition Factors of Angiogenesis after 4 Weeks of Progressive Resistant Training in Sedentary Men. Intern Med Today. 2016; 22 (4) :267-274
39.  Karami S. The effect of glutamine supplementation and periodic training on HSP72 response in soccer players. Master of Science Degree in Sport Physiology, Shahid Rajaee University, Tehran, Iran. [in Persian]
40.  Karami S, Rajabi H. The Effect of Resistance Training on Selected Hemodynamic and Functional Factors of the Older Adults Residents of a Nursing Home in Kahrizak. Salmand: Iranian Journal of Ageing. Forthcoming 2024. [in Persian]
41.   AghaJani A, Alikhani H, Hojjati Z, Gharaat MA. Comparison of the effects of continuous and high intensity interval training on aerobic performance in elite male rowers. J Practical Studies Biosci in Sport. 2016; 4: 7, 23-32. [in Persian]

  • تاریخ دریافت 08 اسفند 1404
  • تاریخ بازنگری 25 خرداد 1405
  • تاریخ پذیرش 31 خرداد 1405
  • تاریخ اولین انتشار 31 خرداد 1405
  • تاریخ انتشار 10 تیر 1405